Advanced Diagnostic Testing for Immune and Inflammatory Conditions
( CIRS )
Comprehensive Testing for Precision Health Monitoring and Personalized Care
HumanBiomics
Test Details
Test |
Collection Tube |
S&H |
Sample Type |
---|---|---|---|
TGF-beta 1 | ACD Acid Citric Dextrose | Frozen/ -20°C | Plasma |
MMP-9 | Gel Separation tube | Frozen/ -20°C | Serum |
VEGF-A | K2EDTA or Gel tube | Refrigerated/ 4°C | Plasma or Serum |
Leptin | K2EDTA or Gel tube | Refrigerated/ 4°C | Plasma or Serum |
Osmolality | K2EDTA or Gel tube | Frozen/ -20°C | Plasma or Serum |
alpha MSH | K2EDTA or Gel tube | Frozen/ -20°C | Plasma or Serum |
ADH | K2EDTA or Gel tube | Frozen/ -20°C | Plasma or Serum |
C4a Fragment | K2EDTA Lavender tube | FedEx Overnight, Dry Ice | Plasma |
C3a Fragment | K2EDTA Lavender tube | FedEx Overnight, Dry Ice | Plasma |
Click more Info
TGF-beta 1 is crucial for tissue regeneration, cell differentiation, embryonic development, and immune modulation. It is found in hematopoietic tissue and activates a pathway that inhibits early cancer cell progression.
TGF-beta 1 aids in extracellular matrix deposition, wound healing, and cirrhosis development. It is produced by various cells, and most have specific receptors for it.
Preferred Specimen(s)
1 mL platelet-free plasma collected ACD Acid Critic Dextrose (Yellow tube) tube
Minimum Volume:0.25 mL
Collection Instructions
Collect in ACD tube (Yellow Top) tube and immediately centrifuge to prepare platelet-poor plasma. Decant the plasma into another pour-over tube. Centrifuge a second time and pour over plasma. Freeze immediately. Centrifuge for 15 minutes at 1000 X g within 30 minutes of collection. Centrifuge plasma again at 3000 X g for 10 minutes for complete platelet removal. Freeze Immediately.
For fixed speed centrifuges such as 645e: Collect in ACD tube (lYellow-top) tube. Centrifuge 3 times for 10 minutes at 1600 X g while decanting the plasma each time before the next spin within 30 minutes of collection. Freeze immediately.
Transport Container: Transport tube Freeze plasma
Transport Temperature: Refrigerated (cold packs)
Specimen Stability
Room temperature: Not established
Refrigerated: 48 hours
Frozen -20° C: 14 days
Frozen -70° C: 30 days
Reject Criteria:
- Hemolysis
- Grossly lipemic
- Specimens with particulate matter or microbial contamination
- Specimens outside of listed stability
Alpha MSH is a 13-amino acid peptide (1665 kDa) derived from pro-opiomelanocorticotropin, sharing its sequence with ACTH 1-13. It stimulates melanin production and melanosome dispersion in skin melanocytes, and also promotes aldosterone synthesis.
Plasma alpha MSH levels increase during endotoxin-induced fever and are elevated in insulin-resistant obese men and AIDS patients.
Preferred Specimen(s)
3 mL frozen plasma collected in an EDTA (lavender-top) tube
Minimum Volume
1 mL
Transport Container
Transport tube
Freeze plasma or serum before shipping
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 90 days
Reject Criteria
Hemolysis • Grossly lipemic • Specimens with particulate matter or microbial contamination • Specimens outside of listed stability
The Vascular Endothelial Growth Factor (VEGF) ELISA is designated solely for research purposes. It may hold significance in assessing patients with different cancer types who are candidates for anti-angiogenesis therapy.
Preferred Specimen(s)
1 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
0.3 mL
Collection Instructions
Mix the sample and centrifuge immediately after collection to separate plasma from cells. Transfer plasma to a plastic specimen transport container and mark the specimen type as plasma on the container. Freeze immediately. Cytokine levels may demonstrate diurnal variation. Recommend cytokine levels be determined at the same time of day for improved longitudinal comparison.
Transport Container
Transport tube
Freeze plasma or serum before shipping
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 4 hours
Refrigerated: 48
hours Frozen: 1 year
Reject Criteria
Hemolysis • Grossly lipemic • Specimens with particulate matter or microbial contamination • Specimens outside of listed stability
MMP participates in inflammation, tissue remodeling, wound healing, and cytokine processing. The expression of MMP-9 is associated with aberrant collagen deposition in pancreatic cancer, as well as the metastasis to lymph nodes by human breast cancer cells and the invasion of regional arteries in giant cell tumors of bone.
Preferred Specimen(s)
1 mL serum
Minimum Volume
0.5 mL
Specimen Container
Plastic screw-cap vial
Freeze plasma or serum before shipping
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 4 hours
Refrigerated: 24 hours
Frozen: 30 days
Reject Criteria
Hemolysis • Grossly lipemic • Specimens with particulate matter or microbial contamination • Specimens outside of listed stability
Collect:
Plasma from a lavender top (K2 - EDTA) tube is the only acceptable sample type. Mix sample thoroughly. Keep tubes cold on wet ice or with a cold pack after collection.
Patient Prep:
Within 30 minutes of venipuncture, centrifuge the collection tube at 1600 (±200) x g (rcf) at 4°C (±2°C) for 15 (±1) minutes. Transfer the cell-free plasma to a clean tube and immediately store samples at -20°C, or freeze at -60°C or below in an ultra-low freezer.
Preferred volume:
1 mL Plasma EDTA
Stability:
-20°C - up to 4 weeks, -60°C or below in an ultra low freezer - up to 2 years
Centrifugation:
Ambient temperature for centrifugation is acceptable provided that the time between collection and storage is minimized (approximately 30 minutes).
Pediatric Collection:
Minimum volume: 0.5 mL plasma, frozen.
Unacceptable Conditions:
Thawed specimen or specimens stored at room temperature prior to shipment.
Storage Transport Temp:
Send frozen plasma Priority Overnight via FedEx and in a well-insulated container on dry ice.
Stability:
Plasma EDTA Stability: -20°C - up to 4 weeks, -60°C or below in an ultra low freezer - up to 2 years
Notes:
Additional testing time may be needed for less than perfect samples or confirmatory testing/reflex testing.
Method: ELISA
Synonyms:
C4a Level, C4ades Arg Level, C4ades Arg Level (C4a Level), C4 (C4a Level), C4 anaphylatoxin (C4a Level), C4a anaphylatoxin, Complement (C4a Level), C4AL
Collection and Shipping: C3a
Collect:
Plasma from a lavender top (K2 - EDTA) tube is the only acceptable sample type. Mix sample thoroughly. Keep tubes cold on wet ice or with a cold pack after collection.
Patient Prep:
Within 30 minutes of venipuncture, centrifuge the collection tube at 1600 (±200) x g (rcf) at 4°C (± 2°C) for 15 (±1) minutes. Transfer the cell-free plasma to a clean tube and immediately store samples at -20°C, or freeze at -60°C or below in an ultra-low freezer.
Preferred volume: 1 mL Plasma EDTA
Stability:
-20°C - up to 4 weeks, -60°C or below in an ultra low freezer - up to 2 years
Centrifugation:
Ambient temperature for centrifugation is acceptable provided that the time between collection and storage is minimized (approximately 30 minutes).
Pediatric Collection:
Minimum volume: 0.5 mL plasma, frozen.
Unacceptable Conditions:
Thawed specimen or specimens stored at room temperature prior to shipment.
Storage Transport Temp:
Send frozen plasma Priority Overnight via FedEx and in a well-insulated container on dry ice.
Stability:
Plasma EDTA Stability: -20°C - up to 4 weeks, -60°C or below in an ultra low freezer - up to 2 years
Notes:
Additional testing time may be needed for less than perfect samples or confirmatory testing/reflex testing.
Method: ELISA
Synonyms:
C3 (C3a Level), C3a anaphylatoxin, C3 anaphylatoxin (C3a Level), Radio Immuno Assay (C3a Level), Complement (C3a Level), C3ades Arg Level (C3a Level), C3a des Arg Level, C3AL
Methodology
Electrochemiluminescence (ECL)
Assay Category
It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Reference Range(s)
Pediatric male and female
5-9.9 Years 0.6-16.8 ng/mL
10-13.9 Years 1.4-16.5 ng/mL
14-17.9 Years 0.6-24.9 ng/mL
Adult Lean Subjects (18-71 Years) with BMI range of 18-25
Male 0.3-13.4 ng/mL
Female 4.7-23.7 ng/mL
Adult Subjects (19-60 Years) with BMI range of 25-30
Male 1.8-19.9 ng/mL
Female 8.0-38.9 ng/mL
Preferred Specimen(s)
1 mL serum
Minimum Volume
0.2 mL
Transport Container
Transport tube
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 8 hours
Refrigerated: 14 days
Frozen: 35 days
Reject Criteria
Moderate hemolysis • Gross hemolysis • Hypericteric specimens
Preferred Specimen
1 mL serum
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in: EDTA (lavender-top) tube or sodium heparin (green-top) tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis
Methodology
Immunofluorescence Assay (IFA)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Reference Range
0-2 Years Not Established
3-17 Years 2.4-26.0 pmol/L
≥18 Years ≤13.7 pmol/L
Specimen Requirements
Serum or plasma
Volume
2 mL
Minimum Volume
0.2 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube
gel-barrier tube or green-top (heparin) tube
Collection
Pediatric: Blood drawn from heelstick for capillary. Separate serum or plasma from cells as soon as possible after clot formation. Transfer specimen to a plastic transport tube.
Storage Instructions
Refrigerate
Stability Requirements
Temperature and Period
Room temperature 14 days
Refrigerated 14 days
Frozen 14 days
Freeze/thaw cycles Stable x3
Causes for Rejection
Hemolysis; EDTA plasma submitted
Test Details
Evaluate electrolyte and water balance, hyperosmolar status, and hydration status; evaluate dehydration, acid-base balance; evaluate seizures; clue to alcoholism, methanol toxicity, ethylene glycol ingestion; evaluate antidiuretic hormone function, liver disease, hyperosmolar coma, evaluate hypernatremia. Osmolarity measures the concentration of particles in solution.
Methodology
Freezing point depression
Reference Interval
Neonatal: may be as low as 266 mOsm/kg
0 to 60 years: 275−295 mOsm/kg
61 years and older: 280−301 mOsm/kg
Information
High serum osmolality can result from hypernatremia, dehydration, hyperglycemia, mannitol therapy, azotemia, ingestion of ethanol, methanol, ethylene glycol. Thus, osmolality has a role in toxicology and in coma evaluation. Elevated serum osmolality with normal sodium suggests possible hyperglycemia, uremia, or alcoholism.
Low serum osmolality may be secondary to overhydration, hyponatremia, syndrome of inappropriate antidiuretic hormone secretion (SIADH) with carcinoma of lung and other entities.
Notes
Weisberg HF. Unraveling the laboratory model of a syndrome: The osmolality model. In: Young DS, Hicks J, Nipper H, et al, eds. Clinician and Chemist. The Relationship of the Laboratory to the Physician. Washington, DC: American Association of Clinical Chemistry;1979:200-243.
Dr. Shoemaker’s analysis of international gene registries, corroborated by case-controlled studies, indicates that 24% of the population exhibits a “mold susceptible” HLA haplotype. This susceptibility puts them at risk of developing chronic biotoxin-related illnesses from exposure to water-damaged buildings.
Additionally, he observed that 21% of the population has “Lyme susceptible” HLA haplotype, making them more prone to developing chronic illness following exposure to biotoxins after contracting Lyme disease. Depending on their unique HLA gene combinations, individuals may be susceptible to one or more biotoxin-related illnesses.
Why Consider These Tests?
These advanced diagnostic tests are crucial for individuals seeking to:
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Monitor Immune Health: Detect and track immune system function, helping to identify imbalances that may lead to chronic conditions.
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Address Inflammatory Issues: Pinpoint the root causes of inflammation in the body, aiding in early diagnosis and more effective treatment plans for various inflammatory diseases.
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Detect Genetic Predispositions: Identify genetic markers that could influence how your body responds to environmental factors, infections, or allergens.
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Support Chronic Illness Management: These tests are valuable tools for patients suffering from long-term conditions like autoimmune diseases, helping healthcare providers tailor treatments.
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Guide Personalized Therapies: By understanding specific biomarkers like TGF-β1 and VEGF, your healthcare team can create more personalized and targeted interventions to improve health outcomes.
Gain insights into chronic conditions such as cardiovascular and autoimmune diseases.
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